@article {561726,
	title = {Limb development and evolution: a frog embryo with no apical ectodermal ridge (AER)},
	journal = {Journal of Anatomy},
	volume = {192},
	year = {1998},
	note = {<p>3100XVTimes Cited:33Cited References Count:51</p>},
	month = {Apr},
	pages = {379-390},
	abstract = {<p>The treefrog Eleutlrerodactylus coqui is a direct developer-it has no tadpole stage. The limb buds develop earlier than in metamorphosing species (indirect developers, such as Xenopus laevis). Previous molecular studies suggest that at least some mechanisms of limb development in E. coqui are similar to those of other vertebrates and we wished to see how limb morphogenesis in this species compares with that in other vertebrates. We found that the hind limb buds are larger and more advanced than the forelimbs at all stages examined, thus differing from the typical amniote pattern. The limb buds were also small compared to those in the chick. Scanning and transmission electron microscopy showed that although the apical ectoderm is thickened, there was no apical ectodermal ridge (AER). In addition, the limb buds lacked the dorsoventral flattening seen in many amniotes. These findings could suggest a mechanical function for the AER in maintaining dorsoventral flattening, although not all data are consistent with this view. Removal of distal ectoderm from E. coqui hindlimb buds does not stop outgrowth, although it does produce anterior defects in the skeletal pattern. The defects are less severe when the excisions are performed earlier. These results contrast with the chick, in which AER excision leads to loss of distal structures. We suggest that an AER was present in the common ancestor of anurans and amniotes and has been lost in at least some direct developers including E. coqui.</p>},
	keywords = {anurans, bud, direct development, distal-less gene, eleutherodactylus, eleutherodactylus-coqui anura, expression, fgf-4, heterochrony, leptodactylidae, limb bud, xenopus},
	isbn = {0021-8782},
	author = {Richardson, M. K. and Carl, T. F. and Hanken, J. and Elinson, R. P. and Cope, C. and Bagley, P.}
}
