Publications

2010
Wu, Y.K., Jiang, K. & Hanken, J., 2010. A new species of newt of the genus Paramesotriton (Salamandridae) from southwestern Guangdong, China, with a new northern record of P. longliensis from western Hubei. Zootaxa , pp. 45-58.Abstract

We report two previously unknown populations of Asian warty newts (Salamandridae: Paramesotriton) in China. The first population, from southwestern Guangdong, is described as a new species, which is closely related to P. guangxiensis based on morphological and molecular data. The second new population, from western Hubei, is assigned to P. longliensis, which extends the known range of this species 400 km northwards. Limited genetic differentiation between P. longliensis and P. zhijinensis suggests that these two names may refer to the same (single) species.

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Kerney, R., Hall, B.K. & Hanken, J., 2010. Regulatory elements of Xenopus col2a1 drive cartilaginous gene expression in transgenic frogs. International Journal of Developmental Biology , 54 , pp. 141-150.Abstract

This study characterizes regulatory elements of collagen2 alpha 7 (col2a1) in Xenopus that enable transgene expression in cartilage-forming chondrocytes. The reporters described in this study drive strong cartilage-specific gene expression, which will be a valuable tool for further investigations of Xenopus skeletal development. While endogenous col2a1 mRNA is expressed in many embryonic tissues, its expression becomes restricted to tadpole and adult chondrocytes. This chondrocyte-specific expression is recapitulated by col2a1 reporter constructs, which were tested through I-Scel meganuclease-mediated transgenesis. These constructs contain a portion of the Xenopus tropicalis col2a1 intron, which aligns to a cartilage-specific intronic enhancer that has been well characterized in mammals. Two overlapping regions of the first intron that are 1.5-Kb and 665-bp long, both of which contain this enhancer sequence, drove EGFP expression in both larval and adult chondrocytes when connected to an upstream promoter. However, neither a truncated 155-bp region that also contains the enhancer, nor a separate 347-bp intronic region that lacks it, was able to drive cartilaginous transgene expression. The two cartilage-specific transgenes are heritable in F1 progeny, which exhibit none of the background expression observed in the injected founders. This study is the first to use the I-Scel technique to characterize an enhancer element in Xenopus, and the first to generate chondrocyte-specific gene expression in a non-mammalian vertebrate. The creation of novel cartilage-specific gene expression provides a new tool for further studies of anuran skeletal development.

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2009
Hanken, J., 2009. David B. Wake. In M. Ruse & J. Travis, ed. Evolution: The First Four Billion Years. Cambridge. Cambridge: Harvard University Press, pp. 901-902.
Hanken, J., 2009. Rx for human (and planetary) health [review of E. Chivian and A Bernstein, eds.; Sustaining Life: How Human Health Depends on Biodiversity. ReVista [Harvard Review of Latin America] , 8 , pp. 64-66.
Wu, Y.K., et al., 2009. A new species of the genus Paramesotriton (Caudata: Salamandridae) from Guangxi Zhuang Autonomous Region, southern China. Zootaxa , pp. 59-68.Abstract

A new species of Asian warty newt, Paramesotriton ermizhaoi, is described from Guangxi Zhuang Autonomous Region in southern China. This species is easily distinguished from other congeners by external morphology and osteology. Phylogenetic analyses based on mitochondrial data place P. ermizhaoi as a possible sister taxon to P. hongkongensis and P. chinensis. The number of trunk vertebrae varies from 12 to 13 in the new species. The relationship between P. ermizhaoi and a potentially new Pachytriton species (Pachytriton C), and the Guangxi population of P. chinensis is briefly discussed.

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2008
Wake, D., Hanken, J. & Bolaños, F., 2008. Bolitoglossa bramei. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Hanken, J., Ibáñez, R. & Wake, D., 2008. Bolitoglossa copia. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Wake, D., Hanken, J. & Bolaños, F., 2008. Bolitoglossa gomezi. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Hanken, J., Ibáñez, R. & Wake, D., 2008. Bolitoglossa magnifica. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Hanken, J., Wake, D. & Bolaños, F., 2008. Bolitoglossa obscura. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Hanken, J., et al., 2008. Bolitoglossa sombra. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Parra-Olea, G., et al., 2008. Chiropterotriton arboreus. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Parra-Olea, G., et al., 2008. Chiropterotriton larvae. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Wake, D., Parra-Olea, G. & Hanken, J., 2008. Pseudoeurycea boneti. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Wake, D., Papenfuss, T. & Hanken, J., 2008. Pseudoeurycea maxima. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Wake, D. & Hanken, J., 2008. Psudoeurycea tlilicxitl. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Parra-Olea, G., et al., 2008. Thorius arboreus. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Parra-Olea, G., et al., 2008. Thorius aureus. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Parra-Olea, G., et al., 2008. Thorius boreas. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version
Parra-Olea, G., et al., 2008. Thorius lunaris. In IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. Publisher's Version

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